rabbit polyclonal anti grp94 antibody  (Proteintech)

Journal: ACS Chemical Neuroscience

Article Title: Improving Proteostasis of Trafficking-Deficient GABA A Receptor Variants by Activating IRE1

doi: 10.1021/acschemneuro.5c00227

Figure Lengend Snippet: Mechanism of action of IRE1/XBP1s activators on enhancing GABA A R variant proteostasis. Certain GABA A R variants misfold in the ER and undergo excessive ER-associated degradation (ERAD), leading to their trafficking deficiency to the plasma membrane. IRE1/XBP1s activators enhance the ER folding capacity to promote the folding of a variant by increasing the interaction between the variant and BiP, a pro-folding chaperone, and further promote the assembly into pentameric receptors in the ER membrane. In addition, such IRE1/XBP1s activators inhibit the ERAD of the variant by decreasing the interaction between the variant and Grp94, Hrd1-Sel1L, and VCP, known ERAD factors for GABA A Rs. Consequently, IRE1/XBP1s activators enhance the anterograde trafficking of the variant to the plasma membrane by increasing the interaction between the variant and LMAN1. The receptor variant is also stabilized after reaching the plasma membrane.

Article Snippet: The rabbit polyclonal anti-Grp94 antibody (#14700-1-AP, 1:250) was obtained from Proteintech.

Techniques: Variant Assay, Clinical Proteomics, Membrane

Journal: Journal of Lipid Research

Article Title: Characterization of a PNLIP variant identified in Amish pediatric patients with congenital pancreatic lipase deficiency

doi: 10.1016/j.jlr.2025.100878

Figure Lengend Snippet: Expression of the PNLIP p.S290N variant induced increased ER stress and the UPR in vitro. HEK 293T cells were transfected to express PNLIP p.S290N and WT. Samples were harvested 68 h post-transfection for immunoblotting and qPCR analysis for ER stress and the UPR markers as described in the Methods. A: Representative immunoblot images for various markers. α-tubulin served as a control. The relative protein abundance for each marker in different cell lysate fractions presented in (A) was quantified relative to WT, with the exception for the insoluble fraction where the values were expressed as relative to S290N. B: Relative BiP protein abundance. C: Relative GRP94 protein abundance. D: Relative mRNA abundance of BiP and CHOP by qPCR. E: A representative image of semi-quantitative PCR analysis of Xbp1 mRNA splicing. F: Quantification of Xbp1 mRNA splicing presented in (E). Mock, empty vector; WT, wild type. The values in quantification graphs are expressed as the mean ± S.D. of 6 or more independent experiments, and One-Way ANOVA Mixed-effects analysis by Tukey's multiple comparisons were performed.

Article Snippet: The following primary antibodies were used: a rabbit polyclonal antibody against human PNLIP generated in our lab (1:5,000) , a rabbit polyclonal antibody against BiP (1:1,000; Cat.: 3183, Cell Signaling), a rabbit polyclonal antibody against GRP94 (1:1,000; Cat.: 2104, Cell Signaling), and a rat monoclonal antibody against α-tubulin (1:2,000; Cat.: sc-53029, Santa Cruz Biotechnology) used as an endogenous control.

Techniques: Expressing, Variant Assay, In Vitro, Transfection, Western Blot, Control, Quantitative Proteomics, Marker, Real-time Polymerase Chain Reaction, Plasmid Preparation

Journal: Cell reports

Article Title: Mechanism and evolutionary origins of alanine-tail C-degron recognition by E3 ligases Pirh2 and CRL2-KLHDC10

doi: 10.1016/j.celrep.2023.113100

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit polyclonal anti-GRP94 , Cell Signaling Technology , Cat# 2104: RRID: AB_823506.

Techniques: Virus, Recombinant, Protease Inhibitor, Amplified Luminescent Proximity Homogenous Assay, Lysis, TA Cloning, Sequencing, Software